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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is implementing a new real-time PCR assay for the direct detection of Chlamydia trachomatis in urine samples. To ensure the accuracy of negative results, it is essential to include:

A) A positive control containing Neisseria gonorrhoeae DNA.
B) An internal amplification control to detect PCR inhibition.
C) A melting curve analysis to confirm the specificity of the amplicon.
D) A high concentration of the Chlamydia trachomatis target sequence.

2. A patient with a history of solid organ transplantation develops diarrhea and abdominal cramps. Stool studies are negative for bacterial pathogens and C. difficile toxins. Colonoscopy reveals characteristic cytomegalic inclusion bodies in colonic biopsies. The MOST likely causative agent is:

A) Norovirus
B) Rotavirus
C) Cytomegalovirus (CMV)
D) Adenovirus

3. For epidemiological tracking and outbreak investigations of Clostridioides difficile infection (CDI), which molecular typing method based on PCR amplification targeting repetitive elements within the pathogenicity locus (PaLoc) has historically been widely used and designated by specific nomenclature (e.g., NAP1/BI
/027)?

A) Pulsed-field gel electrophoresis (PFGE)
B) Whole-genome sequencing (WGS) SNP analysis
C) Multilocus sequence typing (MLST)
D) PCR Ribotyping

4. A patient develops a severe skin infection after a puncture wound sustained while gardening. Culture of the wound exudate grows a Gram-positive, spore-forming bacillus that is anaerobic and produces a potent neurotoxin causing sustained muscle contraction. The MOST likely organism is:

A) Clostridium perfringens
B) Clostridium botulinum
C) Clostridium tetani
D) Bacillus anthracis

5. A patient with HIV infection and a CD4+ T-cell count below 50 cells/µL develops a progressive neurological illness characterized by confusion, motor deficits, and seizures. Brain MRI reveals multiple white matter lesions without mass effect. PCR of the CSF is positive for JC virus. Thepathogenesis of this condition, progressive multifocal leukoencephalopathy (PML), involves:

A) Lytic infection of oligodendrocytes by JC virus, leading to demyelination.
B) Direct cytopathic effect of JC virus on neurons.
C) Opportunistic infection by JC virus due to impaired cell-mediated immunity.
D) Inflammatory demyelination triggered by JC virus infection of astrocytes.

Solutions: